Séminaire de Franck Picard à l'Institut Pasteur

Bonjour à tous,

Frank Picard, chercheur au Laboratoire de Biométrie et Biologie
Evolutive à Lyon, donnera un séminaire à l'Institut Pasteur le 2 avril
2015, à 11h en salle Jean-Paul Aubert.
Vous trouverez ci-dessous le résumé de sa conférence.

Ce séminaire est ouvert aux personnes extérieures à l'Institut, sur
inscription en envoyant un mail à marie-agnes.dillies@pasteur.fr. Merci
de mettre SEMINAIRE en objet. En raison du plan vigipirate, prévoir une
pièce d'identité pour accéder au campus.

A très bientôt,
Marie-Agnès Dillies


High throughput approaches for studying the genomic and epigenetic
landscapes of human replication origins.**

Replication is the mechanism by which genomes are duplicated into two
exact copies. Genomic stability is under the control of a spatiotemporal
program that orchestrates both the positioning and the timing of firing
of about 50,000 replication starting points, also called replication
origins. Replication bubbles found at origins have been very difficult
to map due to their short lifespan. Moreover, with the flood of data
characterizing new sequencing technologies, the precise statistical
analysis of replication data has become an additional challenge. We
propose a new method to map replication origins on the human genome, and
we assess the reliability of our finding using experimental validation
and comparison with origins maps obtained by bubble trapping. This fine
mapping then allowed us to identify potential regulators of the
replication dynamics. Our study highlights the key role of CpG Islands
and identifies new potential epigenetic regulators (methylation of
lysine 4 on histone H4, and tri-methylation of lysine 27 on histone H3)
whose coupling is correlated with an increase in the efficiency of
replication origins, suggesting those marks as potential key regulators
of replication. Overall, our study defines new potentially important
pathways that might regulate the sequential firing of origins during
genome duplication.

--
Marie-Agnès Dillies, PhD
Plate-Forme Transcriptome et Epigénome
Génopole, Institut Pasteur
28 rue du Dr Roux, 75724 Paris cedex 15

Tel: +33(0)1 45 68 86 51 / +33(0)9 54 48 02 79 (on wednesday)
Mobile: +33(0)6 83 29 57 48
Fax: +33(0)1 45 68 84 06